Welcome to the Princess Margaret RNA Medicine Core
The Princess Margaret (PM) RNA Medicine Core is a non-profit academic center specializing in mRNA synthesis, ionizable lipid synthesis, and RNA-based drugs (e.g. siRNA, ASOs, and mRNA) encapsulated lipid nanoparticles(LNP) synthesis. We offer cost-effective, high-quality RNA medicine services. Our advanced facilities and expert team collaborate with academic and industry partners to develop and test new RNA-based therapies.
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We support RNA-LNPs preparation for dry run tests (i.e., 2 µg of mRNA/custom RNAs or 3 nmol of siRNA/ASO), in vitro scale (>= 100 µg of mRNA/custom RNAs and >= 10 nmol of siRNA/ASO), and in vivo scale (> 500 µg of mRNA and > 250 nmol of siRNA/ASO). Our quality control (QC) processes ensure the integrity, purity, and functionality of RNA samples, as well as the particle size, particle uniformity, and encapsulation efficiency of RNA-LNPs in various preparation scales.
Visit our website to learn more about our services, team, and facilities. Whether you’re interested in RNA molecule design, in vitro and in vivo studies, or developing RNA-based therapies, we have the expertise and resources to support your research goals. If you need a service not listed, please inquire!
Our Core
15
Years of Experience
5
Faculties
20+
Specialized Instruments
3
Labs
20+
Publications
Our core offers a range of specialized services to support your RNA-based therapeutics research, from RNA molecule design and synthesis to in vitro and in vivo testing.
RNA Synthesis
Reporter mRNAs
RNA-LNP: What They Are and How They're Made
What are RNA-LNPs?
RNA-LNP stands for RNA-Lipid Nanoparticles - microscopic spherical particles (50-100 nanometers) that encapsulate RNA molecules for therapeutic delivery. These nanoparticles consist of RNA surrounded by a protective lipid shell, enabling safe and effective delivery of genetic medicines to target cells.
LNP Components
RNA Cargo (Inner Core)
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mRNA: Codes for therapeutic proteins
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siRNA: Silences disease-causing genes
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ASO: Modifies gene expression patterns
Lipid Shell (Four Key Lipids)
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Ionizable cationic lipids (40-50%): Enable RNA encapsulation and cellular uptake
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Phospholipids (10-15%): Provide structural stability
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Cholesterol (35-40%): Maintains membrane fluidity
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PEG-lipids (1-3%): Reduce immune recognition and extend circulation
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How RNA-LNPs Are Made
Step 1: Preparation
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Aqueous phase: RNA dissolved in acidic buffer (pH 3-4)
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Organic phase: Lipids dissolved in ethanol
Step 2: Rapid Mixing
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Solutions are mixed rapidly in microfluidic devices
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Flow rate ratio: Typically 3:1 (aqueous:organic)
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Mixing occurs in milliseconds to prevent aggregation
Step 3: Self-Assembly
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pH and ionic strength changes trigger lipid assembly
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RNA becomes encapsulated within forming particles
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Process driven by electrostatic interactions
Step 4: Purification and Concentration
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Dialysis: Remove ethanol and adjust pH to physiological levels
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Ultrafiltration: Concentrate particles and remove free RNA
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Sterile filtration: Remove any large aggregates
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Quality Control Testing
Particle Characterization
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Size distribution: Dynamic light scattering (typically 50-100 nm)
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Polydispersity index: Measure of size uniformity (target <0.2)
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Zeta potential: Surface charge (slightly negative at physiological pH)
RNA Assessment
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Encapsulation efficiency: Percentage of RNA successfully encapsulated (target >80%)
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RNA integrity: Gel electrophoresis to confirm RNA is not degraded
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RNA concentration: Quantitative measurement of total RNA content
Stability Testing
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Storage stability: Particle integrity over time at different temperatures
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Serum stability: Resistance to degradation in biological fluids
Our Partners
